pTurboGFP-mito vector

cat.# FP517

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboGFP-mito FP517 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboGFP Reporter codon usage mammalian Promoter for TurboGFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use green fluorescent labeling of mitochondria

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboGFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TurboGFP coding sequence (ATG): 705-707
Stop codon: 1401-1403
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1557-1562 & 1586-1591
mRNA 3' ends: 1595 & 1607
f1 single-strand DNA origin: 1654-2109
Bacterial promoter for expression of Kan^r gene
-35 region: 2171-2176
-10 region: 2194-2199
Transcription start point: 2206
SV40 origin of replication: 2450-2585
SV40 early promoter
Enhancer (72-bp tandem repeats): 2283-2354 & 2355-2426
21-bp repeats: 2430-2450, 2451-2471 & 2473-2493
Early promoter element: 2506-2512
Major transcription start points: 2502, 2540, 2546 & 2551
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2634-2636
Stop codon: 3426-3428
G->A mutation to remove Pst I site: 2816
C->A (Arg to Ser) mutation to remove BssH II site: 3162
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3664-3669 & 3677-3682
pUC plasmid replication origin: 4013-4656