pTurboGFP-PRL vector

cat.# FP515

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboGFP-PRL FP515 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type promoterless expression vector Reporter TurboGFP Reporter codon usage mammalian Promoter for TurboGFP NO Host cells mammalian, prokaryotic Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Monitoring of activity of different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
TurboGFP
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 793-795
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 949-954 & 978-983
mRNA 3' ends: 987 & 999
f1 single-strand DNA origin: 1046-1501
Bacterial promoter for expression of Kan^r gene
-35 region: 1563-1568
-10 region: 1586-1591
Transcription start point: 1598
SV40 origin of replication: 1842-1977
SV40 early promoter
Enhancer (72-bp tandem repeats): 1675-1746 & 1747-1818
21-bp repeats: 1822-1842, 1843-1863 & 1865-1885
Early promoter element: 1898-1904
Major transcription start points: 1894, 1932, 1938 & 1943
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2026-2028
Stop codon: 2818-2820
G->A mutation to remove Pst I site: 2208
C->A (Arg to Ser) mutation to remove BssH II site: 2554
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3056-3061 & 3069-3074
pUC plasmid replication origin: 3405-4048