pTurboGFP-N vector

cat.# FP512

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboGFP-N FP512 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboGFP Reporter codon usage mammalian Promoter for TurboGFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TurboGFP expression in mammalian cells; generation of fusions to the TurboGFP N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboGFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1375-1377
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1531-1536 & 1560-1565
mRNA 3' ends: 1569 & 1581
f1 single-strand DNA origin: 1628-2083
Bacterial promoter for expression of Kan^r gene
-35 region: 2145-2150
-10 region: 2168-2173
Transcription start point: 2180
SV40 origin of replication: 2424-2559
SV40 early promoter
Enhancer (72-bp tandem repeats): 2257-2328 & 2329-2400
21-bp repeats: 2404-2424, 2425-2445 & 2447-2467
Early promoter element: 2480-2486
Major transcription start points: 2476, 2514, 2520 & 2525
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2608-2610
Stop codon: 3400-3402
G->A mutation to remove Pst I site: 2790
C->A (Arg to Ser) mutation to remove BssH II site: 3136
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3638-3643 & 3651-3656
pUC plasmid replication origin: 3987-4630