pTurboFP635-N vector

cat.# FP722

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboFP635-N FP722 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboFP635 Reporter codon usage mammalian Promoter for TurboFP635 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TurboFP635 expression in mammalian cells; generation of fusions to the TurboFP635 N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboFP635
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1384-1386
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1540-1545 & 1569-1574
mRNA 3' ends: 1578 & 1590
f1 single-strand DNA origin: 1637-2092
Bacterial promoter for expression of Kan^r gene
-35 region: 2154-2159
-10 region: 2177-2182
Transcription start point: 2189
SV40 origin of replication: 2433-2568
SV40 early promoter
Enhancer (72-bp tandem repeats): 2266-2337 & 2338-2409
21-bp repeats: 2413-2433, 2434-2454 & 2456-2476
Early promoter element: 2489-2495
Major transcription start points: 2485, 2523, 2529 & 2534
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2617-2619
Stop codon: 3409-3411
G->A mutation to remove Pst I site: 2799
C->A (Arg to Ser) mutation to remove BssH II site: 3145
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3647-3652 & 3660-3665
pUC plasmid replication origin: 3996-4639