pTurboFP602-mito vector

cat.# FP717

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboFP602-mito FP717 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboFP602 Reporter codon usage mammalian Promoter for TurboFP602 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use true-red fluorescent labeling of mitochondria

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboFP602-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TurboFP602 coding sequence (ATG): 705-707
Stop codon: 1410-1412
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1566-1571 & 1595-1600
mRNA 3' ends: 1604 & 1616
f1 single-strand DNA origin: 1663-2118
Bacterial promoter for expression of Kan^r gene
-35 region: 2180-2185
-10 region: 2203-2208
Transcription start point: 2215
SV40 origin of replication: 2459-2594
SV40 early promoter
Enhancer (72-bp tandem repeats): 2292-2363 & 2364-2435
21-bp repeats: 2439-2459, 2460-2480 & 2482-2502
Early promoter element: 2515-2521
Major transcription start points: 2511, 2549, 2555 & 2560
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2643-2645
Stop codon: 3435-3437
G->A mutation to remove Pst I site: 2825
C->A (Arg to Ser) mutation to remove BssH II site: 3171
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3673-3678 & 3686-3691
pUC plasmid replication origin: 4022-4665