pPhi-Yellow-mito vector

cat.# FP607

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pPhi-Yellow-mito FP607 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter PhiYFP Reporter codon usage mammalian Promoter for PhiYFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use yellow fluorescent labeling of mitochondria

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
PhiYFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of PhiYFP coding sequence (ATG): 705-707
Stop codon: 1407-1409
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1623-1628 & 1652-1657
mRNA 3' ends: 1661 & 1673
f1 single-strand DNA origin: 1720-2175
Bacterial promoter for expression of Kan^r gene
-35 region: 2237-2242
-10 region: 2260-2265
Transcription start point: 2272
SV40 origin of replication: 2516-2651
SV40 early promoter
Enhancer (72-bp tandem repeats): 2349-2420 & 2421-2492
21-bp repeats: 2496-2516, 2517-2537 & 2539-2559
Early promoter element: 2572-2578
Major transcription start points: 2568, 2606, 2612 & 2617
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2700-2702
Stop codon: 3492-3494
G->A mutation to remove Pst I site: 2882
C->A (Arg to Ser) mutation to remove BssH II site: 3228
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3730-3735 & 3743-3748
pUC plasmid replication origin: 4079-4722