pPhi-Yellow-PRL vector

cat.# FP604

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pPhi-Yellow-PRL FP604 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type promoterless expression vector Reporter PhiYFP Reporter codon usage mammalian Promoter for PhiYFP NO Host cells mammalian, prokaryotic Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Monitoring of activity of different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
PhiYFP
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 799-801
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1015-1020 & 1044-1049
mRNA 3' ends: 1053 & 1065
f1 single-strand DNA origin: 1112-1567
Bacterial promoter for expression of Kan^r gene
-35 region: 1629-1634
-10 region: 1652-1657
Transcription start point: 1664
SV40 origin of replication: 1908-2043
SV40 early promoter
Enhancer (72-bp tandem repeats): 1741-1812 & 1813-1884
21-bp repeats: 1888-1908, 1909-1929 & 1931-1951
Early promoter element: 1964-1970
Major transcription start points: 1960, 1998, 2004 & 2009
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2092-2094
Stop codon: 2884-2886
G->A mutation to remove Pst I site: 2274
C->A (Arg to Ser) mutation to remove BssH II site: 2620
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3122-3127 & 3135-3140
pUC plasmid replication origin: 3471-4114