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pPhi-Yellow-PRL vector

cat.# FP604

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pPhi-Yellow-PRLFP60420 μg€ 320 / 160*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typepromoterless expression vector
Reporter codon usagemammalian
Promoter for PhiYFPNO
Host cellsmammalian, prokaryotic
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use Monitoring of activity of different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 799-801
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1015-1020 & 1044-1049
mRNA 3' ends: 1053 & 1065
f1 single-strand DNA origin: 1112-1567
Bacterial promoter for expression of Kanr gene
-35 region: 1629-1634
-10 region: 1652-1657
Transcription start point: 1664
SV40 origin of replication: 1908-2043
SV40 early promoter
Enhancer (72-bp tandem repeats): 1741-1812 & 1813-1884
21-bp repeats: 1888-1908, 1909-1929 & 1931-1951
Early promoter element: 1964-1970
Major transcription start points: 1960, 1998, 2004 & 2009
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2092-2094
Stop codon: 2884-2886
G->A mutation to remove Pst I site: 2274
C->A (Arg to Ser) mutation to remove BssH II site: 2620
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3122-3127 & 3135-3140
pUC plasmid replication origin: 3471-4114

Notice to Purchaser:

PhiYFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,951,923; European Pat. 03779067; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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