pPhi-Yellow-N vector

cat.# FP602

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pPhi-Yellow-N FP602 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter PhiYFP Reporter codon usage mammalian Promoter for PhiYFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use PhiYFP expression in mammalian cells; generation of fusions to the PhiYFP N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
PhiYFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1381-1383
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1597-1602 & 1626-1631
mRNA 3' ends: 1635 & 1647
f1 single-strand DNA origin: 1694-2149
Bacterial promoter for expression of Kan^r gene
-35 region: 2211-2216
-10 region: 2234-2239
Transcription start point: 2246
SV40 origin of replication: 2490-2625
SV40 early promoter
Enhancer (72-bp tandem repeats): 2323-2394 & 2395-2466
21-bp repeats: 2470-2490, 2491-2511 & 2513-2533
Early promoter element: 2546-2552
Major transcription start points: 2542, 2580, 2586 & 2591
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2674-2676
Stop codon: 3466-3468
G->A mutation to remove Pst I site: 2856
C->A (Arg to Ser) mutation to remove BssH II site: 3202
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3704-3709 & 3717-3722
pUC plasmid replication origin: 4053-4696