Tersus polymerase and Tersus Plus PCR kit

cat.# PK123S, PK123L, PK121

- High fidelity
- High specificity
- Fast antibody-based hot start
- TA cloning compatibility

Tersus polymerase is a specially developed mix of proofreading and highly processive PCR enzymes and hot start antibodies that inhibit polymerase activity at room temperature, preventing non-specific amplification and allowing flexible reaction setup. Tersus polymerase has about 4 times lower error rate than Encyclo polymerase that makes it an ideal choice for cloning and other applications requiring high-fidelity amplification. High specificity of Tersus polymerase en-sures its excellent performance in amplification of difficult templates, such as highly homologous repeats, genomic DNA or cDNA libraries. Tersus polymerase provides robust amplification of DNA fragments up to 3 kb in length.

Applications:

- Amplification of DNA for subsequent cloning or sequencing
- Site-specific mutagenesis
- High specific PCR from complex templates
- Real-time PCR with intercalating dyes (SYBR Green I etc.)

	Tersus polymerase provides highly specific amplification of difficult templates. A 230 bp fragment of L1Hs repeat was amplified from 5 ng of human genomic DNA using different polymerases. M - 1 kb DNA ladder; 1 – Taq polymerase; 2 – Encyclo polymerase; 3 – Tersus polymerase, 28 cycles.

	PCR amplification of phage λ DNA fragments using Tersus polymerase. DNA fragments were amplified from 25 ng of template in 15 cycles (wells 1 and 2) and 19 cycles (wells 3-5). 1 – 3 kb fragment; 2 – 5 kb fragment; 3 – 8 kb fragment; 4 – 10 kb fragment; 5 – 15 kb fragment; M – 1 kb DNA ladder.

Possible limitations:

- Not recommended for amplification of long (>3 kb) templates (use Encyclo polymerase instead)
- Not suitable for real-time PCR methods based on DNA probe breakdown (such as TaqMan) due to the lack of 5’>3’ exonuclease activity
- Not recommended for allele-specific SNP detection due to the presence of proofreading 3’>5’ exonuclease activity

Available products

PRODUCT	CAT.#	DESCRIPTION	SIZE	PRICE	USER MANUAL (PDF)
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.
Tersus polymerase	PK123S	Tersus polymerase, Tersus Plus buffer	200 x 25 μl reactions	€ 120
	PK123L		1000 x 25 μl reactions	€ 470
Tersus Plus PCR kit	PK121	Tersus polymerase, dNTPs, Tersus Plus buffer, Tersus GC buffer, Tersus Red buffer, sterile PCR water	100 x 50 μl reactions or 200 x 25 μl reactions	€ 150

Shipping and Storage conditions: The products must be shipped and stored at -20°C.