Encyclo polymerase and Encyclo Plus PCR kit

cat.# PK002S, PK002L, PK101

- High processivity
- Proofreading 3'>5' exonuclease activity
- Fast antibody-based hot start
- TA cloning compatibility

Encyclo polymerase produces high yields of PCR products from a wide variety of templates and is suitable for most PCR and primer extension applications, including the amplification of difficult templates and long PCR. It is especially recommended for cDNA amplification because of optimal combination of high fidelity and processivity provided by Encyclo polymerase mix.

Encyclo Plus PCR kit includes all components necessary for PCR: Encyclo polymerase mix, a mix of high purity deoxyribonucleotides and sterile PCR water. The kit includes three reaction buffers: general purpose Encyclo buffer, Encyclo GC buffer for amplification of GC-rich templates and Encyclo Red buffer for direct loading on agarose gels.

Applications:

- cDNA amplification
- Long PCR (up to 15 Kb)
- Amplification of low-copy-number targets
- Routing PCR, real-time PCR with intercalating dyes (SYBR Green I etc.)

	PCR amplification of phage λ DNA fragments using Encyclo Plus PCR kit. Fragment length is indicated at the top. M – 50 ng of DNA size markers (1 kb DNA ladder, NEB).

PCR amplification of first-strand cDNA samples (prepared from total RNA from different tissues) using Encyclo Plus PCR kit. 1 – mouse liver; 2 – mouse skeletal muscle; 3 – mouse brain; 4 – human leucocytes; 5 – human lung; 6 – human skeletal muscle; 7 – mosquito grub; 8 – copepod Pontella sp.; 9 – tomato Lycopersicon esculentum. M – 1 kb DNA size marker, SibEnzyme, Russia.

Possible limitations:

- not suitable for real-time PCR methods based on DNA probe breakdown (such as TaqMan) due to the lack of 5'>3' exonuclease activity
- not recommended for allele-specific SNP detection due to the presence of proofreading 3'>5' exonuclease activity

Available products

PRODUCT	CAT.#	DESCRIPTION	SIZE	PRICE	USER MANUAL (PDF)
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.
Encyclo polymerase	PK002S	Encyclo polymerase, Encyclo buffer	200 x 25 μl reactions	€ 110
	PK002L		1000 x 25 μl reactions	€ 440
Encyclo Plus PCR kit	PK101	Encyclo polymerase, dNTPs, Encyclo buffer, Encyclo GC buffer, Encyclo Red buffer, sterile PCR water	100 x 50 μl reactions or 200 x 25 μl reactions	€ 140

Shipping and Storage conditions: The products must be shipped and stored at -20°C.