TagYFP normalized excitation (thin line) and emission (thick line) spectra.
Spectra viewer tool Download TagYFP spectra (xls)
| | CHARACTERISTIC | |
|---|
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 27.0 | | Polypeptide length, aa | 239 | | Fluorescence color | yellow | | Excitation maximum, nm | 508 | | Emission maximum, nm | 524 | | Quantum yield | 0.62 | | Extinction coefficient, M-1cm-1 | 50 000 | | Brightness* | 31 | | Brightness, % of EGFP | 94 | | pKa | 5.5 | | Structure | monomer | | Aggregation | no | | Maturation rate at 37°C | fast | | Photostability | high | | Cell toxicity | not observed | | Main advantages | bright yellow monomeric fluorescent protein |
|
|---|