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    PS-CFP2

Cyan-to-green photoswitchable fluorescent protein PS-CFP2

- Monomer, succesful perfomance in fusions
- Irreversible photoconversion from a cyan to a green fluorescent form
- High contrast of photoconversion
- High pH stability allowing labeling of acidic organelles
- Recommended for tracking cell, organelle, and protein movement, monitoring the protein turnover and superresolution imaging

Main properties

PS-CFP2 spectra

Normalized excitation (thin line) and emission (thick line) for non-activated (cyan) and activated (green) spectra.

Download PS-CFP2 spectra (xls)

CHARACTERISTICbefore  /  after photoactivation
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Fluorescence colorcyan   /   green
Excitation maximum, nm400   /   490
Emission maximum, nm468   /   511
Quantum yield0.20   /   0.23
Extinction coefficient, M-1cm-143 000   /   47 000
Brightness*8.6   /   10.8
pKa4.3   /   6.1
Activating lightUV-violet (e.g. 405 nm)
Calculated contrast, foldup to 2000
Structuremonomer
Cell toxicitynot observed
Aggregationno
Maturation rate at 37°Cfast
Molecular weight, kDa27
Polypeptide length, aa238
Main advantagesBright and pH stable routin cyan label, monomeric high-contrast irreversibly photoswitchable tag suitable for protein tracking
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