Kindling red fluorescent protein KFP-Red

- Reversible or irreversible photoactivation
- Activated by green light that does not damage cells and tissues
- Quenching by blue light
- Recommended for tracking cells and cellular organelle movements

Main properties

KFP-Red normalized spectra. Download KFP-Red spectra (xls)

CHARACTERISTIC before  /  after photoactivation * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Fluorescence color NO   /   red Excitation maximum, nm 580   /   580 Emission maximum, nm 600   /   600 Quantum yield <0.001   /   0.07 Extinction coefficient, M-1cm-1 123 000   /   59 000 Brightness* 0   /   4.1 Activating light green (530-560 nm) Calculated contrast, fold 35-70 Structure tetramer Cell toxicity not observed Aggregation no Maturation rate at 37°C medium Molecular weight, kDa 26 Polypeptide length, aa 238 Main advantages Capable of reversible or irreversible kindling by green light irradiation Possible limitations Limited applicability for fusions generation