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Green fluorescent protein CopGFP
- Bright green fluorescence
- Extra fast maturation at a wide range of temperatures
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CopGFP (ppluGFP2 in our scientific publications, Shagin et al., 2004) is a green fluorescent protein cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). CopGFP is characterized by superbright green fluorescence (excitation/ emission max = 482/ 502 nm) and fast maturation rate at a wide range of temperatures.
CopGFP demonstrates successful performance when expressed in cold-blooded animals, however exhibits a tendency to form aggregates in mammalian cells especially in long-term cultures.
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Main properties
CopGFP normalized excitation (thin line) and emission (thick line) spectra.
Download CopGFP spectra (xls)
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 26 | | Fluorescence color | green | | Excitation maximum, nm | 482 | | Emission maximum, nm | 502 | | Quantum yield | 0.60 | | Extinction coefficient, M-1cm-1 | 70000 | | Brightness* | 42.0 | | Brightness, % of EGFP | 126 | | pKa | 4.3 | | Structure | tetramer | | Aggregation | yes | | Maturation rate at 37°C | super fast | | Photostability | high | | Cell toxicity | not observed |
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Recommended filter sets and antibodies
CopGFP can be recognized using Anti-TurboGFP(d) (Cat.# AB513) antibodies available from Evrogen.
CopGFP can be detected using common fluorescence filter sets for EGFP, FITC, and other green dyes. Recommended Omega Optical filter sets are QMAX-Green, XF100-2, XF100-3, XF115-2, and XF116-2.
References:
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Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV.
GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity.
Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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