Caspase-3 apoptosis sensor Casper3-GR

- Early detection of Caspase-3 activity onset
- High sensitivity
- Direct expression in cells
- No exogenous chemical compounds required
- Proven suitability for FLIM-based screenings
- Recommended for early detection of apoptosis

Main properties

Excitation (thin lines) and emission (thick lines) spectra of TagGFP (green) and TagRFP (red) are shown individually. Spectral overlap is filled with gray. Download Casper3-GR spectra (xls)

CHARACTERISTIC   FRET donor TagGFP     Fluorescence color green     Excitation maximum, nm 482     Emission maximum, nm 505     Brightness, % of EGFP 104     pKa 4.7 FRET acceptor TagRFP     Fluorescence color red (orange)     Excitation maximum, nm 555     Emission maximum, nm 584     Brightness, % of EGFP 148     pKa 3.8 Calculated Förster distance R0 5.70 FRET efficiency E 0.50 Specificity caspase-3 activity Response elimination of FRET Polypeptide length, aa 484 Molecular weight, kDa 54