cDNA synthesis

cDNA synthesis service

cat.# CS030

- High-quality full-length-enriched amplified cDNA for various applications including next generation sequencing (Roche 454, Illumina Solexa, ABI SOLiD)
- From limited amounts of total or poly(A)+ RNA

Full-length-enriched double stranded cDNA is produced using modified template-switching (SMART) approach. The method combines cDNA synthesis and amplification and allows generating representative cDNA populations enriched with full-length sequences from as little as 0.1 micrograms of RNA. For making first strand cDNA, different primers can be used depending on project requirements.

Click here for information on types of cDNA preps and prices.

The cDNA can be used for:

  • Sequencing using next generation technologies (Roche 454, Illumina Solexa, ABI SOLiD)
  • Construction of representative cDNA libraries
  • cDNA subtractive hybridization
  • cDNA normalization
  • Isolation of full-length cDNAs by RACE
  • PCR cloning / array development
  • Virtual Northern blot

Typical result of cDNA synthesis

(A) Amplified cDNA sample prepared from human placenta total RNA. M – 1-kb DNA ladder, Sibenzyme.

(B) Amplified cDNA sample prepared from total RNA of freshwater planarian. M – 1-kb DNA ladder, GibcoBRL.

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