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pmKate2-EB3 vector

cat.# FP316

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pmKate2-EB3FP31620 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for mKate2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use far-red fluorescent labeling of EB3 protein

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
EB3: 652-1494
mKate2: 1516-2015
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2367-2372 & 2396-2401
mRNA 3' ends: 2405 & 2417
f1 single-strand DNA origin: 2464-2919
Bacterial promoter for expression of Kanr gene
-35 region: 2981-2986
-10 region: 3004-3009
Transcription start point: 3016
SV40 origin of replication: 3260-3395
SV40 early promoter
Enhancer (72-bp tandem repeats): 3093-3164 & 3165-3236
21-bp repeats: 3240-3260, 3261-3281 & 3283-3303
Early promoter element: 3316-3322
Major transcription start points: 3312, 3350, 3356 & 3361
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3444-3446
Stop codon: 4236-4238
G->A mutation to remove Pst I site: 3626
C->A (Arg to Ser) mutation to remove BssH II site: 3972
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4474-4479 & 4487-4492
pUC plasmid replication origin: 4823-5466

Notice to Purchaser:

mKate2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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