The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.
|Bgl II||Sac I||Hind III||EcoR I||Sal I||Kpn I||Apa I*||BamH I||Age I||TurboGFP|
|Afe I||Xho I||Pst I*||Sac II||Nco I*|
* – not unique site.
pTurboGFP-PRL is a promoterless vector encoding green fluorescent protein TurboGFP, which can be used as in vivo reporter of promoter activity (see reporter description). TurboGFP codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996]. To increase mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of the TurboGFP coding sequence [Kozak, 1987].
Multiple cloning site (MCS) is located upstream of the Kozak consensus translation initiation site and can be used to clone a promoter or a promoter/enchancer combination of interest. Without the addition of a functional promoter, this vector will not express TurboGFP.
The vector backbone contains SV40 origin for replication in mammalian cells expressing SV40 T-antigen, pUC origin of replication for propagation in
SV40 early promoter (PSV40) provides neomycin resistance gene (Neor) expression to select stably transfected eukaryotic cells using G418. Bacterial promoter (P) provides kanamycin resistance gene expression (Kanr) in
Note: The plasmid DNA was isolated from dam+-methylated
Suitable host strains for propagation in
Location of features
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 793-795
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 949-954 & 978-983
mRNA 3' ends: 987 & 999
f1 single-strand DNA origin: 1046-1501
Bacterial promoter for expression of Kanr gene
-35 region: 1563-1568
-10 region: 1586-1591
Transcription start point: 1598
SV40 origin of replication: 1842-1977
SV40 early promoter
Enhancer (72-bp tandem repeats): 1675-1746 & 1747-1818
21-bp repeats: 1822-1842, 1843-1863 & 1865-1885
Early promoter element: 1898-1904
Major transcription start points: 1894, 1932, 1938 & 1943
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2026-2028
Stop codon: 2818-2820
G->A mutation to remove Pst I site: 2208
C->A (Arg to Ser) mutation to remove BssH II site: 2554
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3056-3061 & 3069-3074
pUC plasmid replication origin: 3405-4048
TurboGFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,678,893; European Pat. 1576157; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.
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