pFusionRed-B

pFusionRed-B vector

cat.# FP438

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pFusionRed-BFP43820 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typebacterial expression vector
ReporterFusionRed
Reporter codon usagemammalian
Promoter for FusionRedT5 promoter/lac operator
Host cellsprokaryotic
Selectionampicillin
ReplicationColE1 ori
Use Source of the FusionRed coding sequence; FusionRed expression in bacterial cells
5'-Region
FusionRed
BamH I
RBS ATG. AGA.GGA.TCG. GGA.TCC. TTA.CCG.GTC.GCC.ACC. ATG.GTG.AGC.GAG...
3'-Region
Stop Eag I Hind III
Not I
... TGA. G CG.GCC.G C A.AGC.TT

Vector description

pFusionRed-B is a prokaryotic expression vector encoding red fluorescent protein FusionRed (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of FusionRed coding sequence. Flanking restriction sites are convenient for excision of FusionRed sequence and its further insertion into other expression vectors of choice. Alternatively, FusionRed coding sequence can be amplified by PCR.

Note: The plasmid DNA was isolated from dam+-methylated E. coli. Therefore some restriction sites are blocked by methylation. If you wish to digest the vector using such sites you will need to transform the vector into a dam- host and make fresh DNA.

The vector can be also used for FusionRed expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.


Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
FusionRed coding sequence: 147-846
Lambda t0 transcriptional termination region: 875-969
rrnB T1 transcriptional termination region: 1731-1829
ColE1 origin of replication: 2305
beta-lactamase coding sequence: 3923-3063


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248

Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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