|
Red fluorescent protein TagRFP
- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications
Available variants and fusions
Variant | Description | Related vector | Cat.# | Click for image |
---|
|
Humanized TagRFP
|
TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
|
pTagRFP-C
|
FP141
|
pTagRFP-N
|
FP142
|
TagRFP-AS variant
|
TagRFP-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clones.
|
Gateway® TagRFP-AS-C entry clone
|
FP148
|
Gateway® TagRFP-AS-N entry clone
|
FP149
|
TagRFP-tubulin fusion
|
Human α-tubulin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-tubulin in living cells.
|
pTagRFP-tubulin
|
FP145
|
|
TagRFP-mito fusion
|
A mitochondrial targeting sequence (MTS) is fused to the TagRFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of mitochondria.
|
pTagRFP-mito
|
FP147
|
|
TagRFP-Cx43 fusion
|
Rat connexin 43 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 43 in living cells.
|
pTagRFP-Cx43
|
FP364
|
|
TagRFP-Golgi fusion
|
Golgi targeting sequence (fragment of human β-1,4-galactosyltransferase) is fused to the TagRFP N-terminus. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of Golgi apparatus.
|
pTagRFP-Golgi
|
FP367
|
|
TagRFP-H2B fusion
|
Human histone H2B is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of histone H2B in living cells.
|
pTagRFP-H2B
|
FP368
|
|
References:
-
Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
-
Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
-
Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
|