TagRFP

Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications


Available variants and fusions
VariantDescriptionRelated vectorCat.#Click for image
Humanized TagRFP TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. pTagRFP-C FP141
pTagRFP-N FP142
TagRFP-AS variant TagRFP-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clones. Gateway® TagRFP-AS-C entry clone FP148
Gateway® TagRFP-AS-N entry clone FP149
TagRFP-actin fusion Human β-actin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of β-actin in living cells. pTagRFP-actin FP144
TagRFP-tubulin fusion Human α-tubulin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-tubulin in living cells. pTagRFP-tubulin FP145
TagRFP-mito fusion A mitochondrial targeting sequence (MTS) is fused to the TagRFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of mitochondria. pTagRFP-mito FP147
TagRFP-actinin fusion Human α-actinin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-actinin in living cells. pTagRFP-actinin FP360
TagRFP-integrin fusion Human α-V-integrin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-V-integrin in living cells. pTagRFP-integrin FP361
TagRFP-Cx26 fusion Rat connexin 26 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 26 in living cells. pTagRFP-Cx26 FP362
TagRFP-Cx32 fusion Human connexin 32 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 32 in living cells. pTagRFP-Cx32 FP363
TagRFP-Cx43 fusion Rat connexin 43 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 43 in living cells. pTagRFP-Cx43 FP364
TagRFP-EB3 fusion Human microtubule-associated EB3 protein is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of EB3 protein in living cells. pTagRFP-EB3 FP365
TagRFP-FAK fusion Chicken focal adhesion kinase is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of focal adhesion kinase in living cells. pTagRFP-FAK FP366
TagRFP-Golgi fusion Golgi targeting sequence (fragment of human β-1,4-galactosyltransferase) is fused to the TagRFP N-terminus. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of Golgi apparatus. pTagRFP-Golgi FP367
TagRFP-H2B fusion Human histone H2B is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of histone H2B in living cells. pTagRFP-H2B FP368
TagRFP-keratin fusion Human cytokeratin-18 is fused to theTagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of cytokeratin-18 in living cells. pTagRFP-keratin FP369
TagRFP-laminB1 fusion Human lamin B1 is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of lamin B1 in living cells. pTagRFP-laminB1 FP370
TagRFP-profilin fusion Mouse profilin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of profilin in living cells. pTagRFP-profilin FP371
TagRFP-vinculin fusion Human vinculin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of vinculin in living cells. pTagRFP-vinculin FP372
TagRFP-zyxin fusion Human zyxin is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of zyxin in living cells. pTagRFP-zyxin FP373


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
  • Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
  • Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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