Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Main properties

TagRFP normalized excitation (thin line) and emission (thick line) spectra. Download TagRFP spectra (xls)

CHARACTERISTIC * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Molecular weight, kDa 27 Polypeptide length, aa 237 Fluorescence color red (orange) Excitation maximum, nm 555 Emission maximum, nm 584 Quantum yield 0.48 Extinction coefficient, M-1cm-1 100 000 Brightness* 48.0 Brightness, % of EGFP 148 pKa 3.8 Structure monomer Aggregation no Maturation rate at 37°C fast Photostability medium Cell toxicity not observed Main advantages bright red monomeric fluorescent protein Possible limitations medium photostability