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Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Main properties

TagRFP spectra

TagRFP normalized excitation (thin line) and emission (thick line) spectra.

Spectra viewer tool
Download TagRFP spectra (xls)

* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
** Time to bleach 50% of fluorescent signal brightness.
Molecular weight, kDa27
Polypeptide length, aa237
Fluorescence colorred (orange)
Excitation maximum, nm555
Emission maximum, nm584
Quantum yield0.48
Extinction coefficient, M-1cm-1100 000
Brightness, % of EGFP148
Maturation half-time, min100
Photostability, widefield**48
Photostability, confocal**125
Cell toxicitynot observed
Main advantagesbright red monomeric fluorescent protein
Possible limitationsmedium photostability
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