TagRFP

Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Main properties

TagRFP spectra

TagRFP normalized excitation (thin line) and emission (thick line) spectra.

Spectra viewer tool
Download TagRFP spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
** Time to bleach 50% of fluorescent signal brightness.
Molecular weight, kDa27
Polypeptide length, aa237
Fluorescence colorred (orange)
Excitation maximum, nm555
Emission maximum, nm584
Quantum yield0.48
Extinction coefficient, M-1cm-1100 000
Brightness*48.0
Brightness, % of EGFP148
pKa3.8
Structuremonomer
Aggregationno
Maturation half-time, min100
Photostability, widefield**48
Photostability, confocal**125
Cell toxicitynot observed
Main advantagesbright red monomeric fluorescent protein
Possible limitationsmedium photostability
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