peTurboYFP-dest1 vector

cat.# FP617

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price peTurboYFP-dest1 FP617 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboYFP Reporter codon usage mammalian Promoter for TurboYFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TurboYFP expression in mammalian cells; generation of fusions to the TurboYFP-dest1 N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboYFP-dest1
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Last amino acid in TurboYFP: 1405-1407
Amino acid residues of mouse ornithine decarboxylase (MODC) PEST sequence: 1429-1548
Stop codon: 1549-1551
Fragment of human beta globin (HBB) gene
Last 35 bp of HBB exon 2: 1560-1594
HBB intron 2: 1595-2445
First 233 bp of HBB exon 3: 2446-2678
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2820-2825 & 2849-2854
mRNA 3' ends: 2858 & 2870
f1 single-strand DNA origin: 2917-3372
Bacterial promoter for expression of Kan^r gene
-35 region: 3434-3439
-10 region: 3457-3462
Transcription start point: 3469
SV40 origin of replication: 3713-3848
SV40 early promoter
Enhancer (72-bp tandem repeats): 3546-3617 & 3618-3689
21-bp repeats: 3693-3713, 3714-3734 & 3736-3756
Early promoter element: 3769-3775
Major transcription start points: 3765, 3803, 3809 & 3814
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3897-3899
Stop codon: 4689-4691
G->A mutation to remove Pst I site: 4079
C->A (Arg to Ser) mutation to remove BssH II site: 4425
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4927-4932 & 4940-4945
pUC plasmid replication origin: 5276-5919