pTurboYFP-N vector

cat.# FP612

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboYFP-N FP612 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboYFP Reporter codon usage mammalian Promoter for TurboYFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TurboYFP expression in mammalian cells; generation of fusions to the TurboYFP N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 592-678
TurboYFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1408-1410
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1564-1569 & 1593-1598
mRNA 3' ends: 1602 & 1614
f1 single-strand DNA origin: 1661-2116
Bacterial promoter for expression of Kan^r gene
-35 region: 2178-2183
-10 region: 2201-2206
Transcription start point: 2213
SV40 origin of replication: 2457-2592
SV40 early promoter
Enhancer (72-bp tandem repeats): 2290-2361 & 2362-2433
21-bp repeats: 2437-2457, 2458-2478 & 2480-2500
Early promoter element: 2513-2519
Major transcription start points: 2509, 2547, 2553 & 2558
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2641-2643
Stop codon: 3433-3435
G->A mutation to remove Pst I site: 2823
C->A (Arg to Ser) mutation to remove BssH II site: 3169
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3671-3676 & 3684-3689
pUC plasmid replication origin: 4020-4663