pTurboRFP-mito vector

cat.# FP237

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboRFP-mito FP237 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboRFP Reporter codon usage mammalian Promoter for TurboRFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red (orange) fluorescent labeling of mitochondria

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboRFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TurboRFP coding sequence (ATG): 705-707
Stop codon (TGA): 1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1554-1559 & 1583-1588
mRNA 3' ends: 1592 & 1604
f1 single-strand DNA origin: 1651-2106
Bacterial promoter for expression of Kan^r gene
-35 region: 2168-2173
-10 region: 2191-2196
Transcription start point: 2203
SV40 origin of replication: 2447-2582
SV40 early promoter
Enhancer (72-bp tandem repeats): 2280-2351 & 2352-2423
21-bp repeats: 2427-2447, 2448-2468 & 2470-2490
Early promoter element: 2503-2509
Major transcription start points: 2499, 2537, 2543 & 2548
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2631-2633
Stop codon: 3423-3425
G->A mutation to remove Pst I site: 2813
C->A (Arg to Ser) mutation to remove BssH II site: 3159
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3661-3666 & 3674-3679
pUC plasmid replication origin: 4010-4653