pTurboRFP-N vector

cat.# FP232

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboRFP-N FP232 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboRFP Reporter codon usage mammalian Promoter for TurboRFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TurboRFP expression in mammalian cells; generation of fusions to the TurboRFP N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
Turbo-RFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1375-1377
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1528-1533 & 1557-1562
mRNA 3' ends: 1566 & 1578
f1 single-strand DNA origin: 1625-2080
Bacterial promoter for expression of Kan^r gene
-35 region: 2142-2147
-10 region: 2165-2170
Transcription start point: 2177
SV40 origin of replication: 2421-2556
SV40 early promoter
Enhancer (72-bp tandem repeats): 2254-2325 & 2326-2397
21-bp repeats: 2401-2421, 2422-2442 & 2444-2464
Early promoter element: 2477-2483
Major transcription start points: 2473, 2511, 2517 & 2522
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2605-2607
Stop codon: 3397-3399
G->A mutation to remove Pst I site: 2787
C->A (Arg to Ser) mutation to remove BssH II site: 3133
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3635-3640 & 3648-3653
pUC plasmid replication origin: 3984-4627