pTurboRFP-B vector

cat.# FP233

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboRFP-B FP233 20 μg € 320 / 160* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type bacterial expression vector Reporter TurboRFP Reporter codon usage mammalian Promoter for TurboRFP T5 promoter/lac operator Host cells prokaryotic Selection ampicillin Replication ColE1 ori Use Source of the TurboRFP coding sequence; TurboRFP expression in bacterial cells

5'-Region

				TurboRFP
			BamH I
RBS	ATG.	AGA.GGA.TCG.	GGA.TCC.	ATG.AGC.GAG...

3'-Region

	Stop
		Hind III
...GGG.CAC.AGA.	TG	A.	AGC.TT

Vector description

pTurboRFP-B is a prokaryotic expression vector encoding red (orange) fluorescent protein TurboRFP (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of TurboRFP coding sequence. Flanking restriction sites are convenient for excision of TurboRFP sequence and its further insertion into other expression vectors of choice. Alternatively, TurboRFP coding sequence can be amplified by PCR.

The vector can be also used for TurboRFP expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.

Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
TurboRFP coding sequence: 132-827
Lambda t0 transcriptional termination region: 848-942
rrnB T1 transcriptional termination region: 1704-1802
ColE1 origin of replication: 2278
beta-lactamase coding sequence: 3896-3036