pTurboFP650-N vector

cat.# FP732

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboFP650-N FP732 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TurboFP650 Reporter codon usage mammalian Promoter for TurboFP650 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use TurboFP650 expression in mammalian cells; generation of fusions to the TurboFP650 N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboFP650
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1381-1383
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1536-1541 & 1565-1570
mRNA 3' ends: 1574 & 1586
f1 single-strand DNA origin: 1633-2088
Bacterial promoter for expression of Kan^r gene
-35 region: 2150-2155
-10 region: 2173-2178
Transcription start point: 2185
SV40 origin of replication: 2429-2564
SV40 early promoter
Enhancer (72-bp tandem repeats): 2262-2333 & 2334-2405
21-bp repeats: 2409-2429, 2430-2450 & 2452-2472
Early promoter element: 2485-2491
Major transcription start points: 2481, 2519, 2525 & 2530
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2613-2615
Stop codon: 3405-3407
G->A mutation to remove Pst I site: 2795
C->A (Arg to Ser) mutation to remove BssH II site: 3141
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3643-3648 & 3656-3661
pUC plasmid replication origin: 3992-4635