pTagYFP-mito vector

cat.# FP137

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagYFP-mito FP137 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagYFP Reporter codon usage mammalian Promoter for TagYFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use yellow fluorescent labeling of mitochondria

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagYFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TagYFP coding sequence (ATG): 705-707
Stop codon: 1422-1424
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1578-1583 & 1607-1612
mRNA 3' ends: 1616 & 1628
f1 single-strand DNA origin: 1675-2130
Bacterial promoter for expression of Kan^r gene
-35 region: 2192-2197
-10 region: 2215-2220
Transcription start point: 2227
SV40 origin of replication: 2471-2606
SV40 early promoter
Enhancer (72-bp tandem repeats): 2304-2375 & 2376-2447
21-bp repeats: 2451-2471, 2472-2492 & 2494-2514
Early promoter element: 2527-2533
Major transcription start points: 2523, 2561, 2567 & 2572
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2655-2657
Stop codon: 3447-3449
G->A mutation to remove Pst I site: 2837
C->A (Arg to Ser) mutation to remove BssH II site: 3183
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3685-3690 & 3698-3703
pUC plasmid replication origin: 4034-4677