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pTagYFP-C vector

cat.# FP131

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pTagYFP-CFP13120 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for TagYFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use TagYFP expression in mammalian cells; generation of fusions to the TagYFP C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1408-1410
Last amino acid in TagYFP: 1327-1329
MCS: 1330-1407
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1550-1555 & 1579-1584
mRNA 3' ends: 1588 & 1600
f1 single-strand DNA origin: 1647-2102
Bacterial promoter for expression of Kanr gene
-35 region: 2164-2169
-10 region: 2187-2192
Transcription start point: 2199
SV40 origin of replication: 2443-2578
SV40 early promoter
Enhancer (72-bp tandem repeats): 2276-2347 & 2348-2419
21-bp repeats: 2423-2443, 2444-2464 & 2466-2486
Early promoter element: 2499-2505
Major transcription start points: 2495, 2533, 2539 & 2544
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2627-2629
Stop codon: 3419-3421
G->A mutation to remove Pst I site: 2809
C->A (Arg to Ser) mutation to remove BssH II site: 3155
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3657-3662 & 3670-3675
pUC plasmid replication origin: 4006-4649

Notice to Purchaser:

TagYFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,888,113; European Pat. 06809023; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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