pTagCFP-actin vector

cat.# FP114

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagCFP-actin FP114 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagCFP Reporter codon usage mammalian Promoter for TagCFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use cyan fluorescent labeling of β-actin filaments

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
TagCFP
Start codon (ATG): 613-615
Last amino acid in TagCFP: 1324-1326
Beta-Actin: 1348-2475
Stop codon: 2473-2475
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2636-2641 & 2665-2670
mRNA 3' ends: 2674 & 2686
f1 single-strand DNA origin: 2733-3188
Bacterial promoter for expression of Kan^r gene
-35 region: 3250-3255
-10 region: 3273-3278
Transcription start point: 3285
SV40 origin of replication: 3529-3664
SV40 early promoter
Enhancer (72-bp tandem repeats): 3362-3433 & 3434-3505
21-bp repeats: 3509-3529, 3530-3550 & 3552-3572
Early promoter element: 3585-3591
Major transcription start points: 3581, 3619, 3625 & 3630
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3713-3715
Stop codon: 4505-4507
G->A mutation to remove Pst I site: 3895
C->A (Arg to Ser) mutation to remove BssH II site: 4241
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4743-4748 & 4756-4761
pUC plasmid replication origin: 5092-5735