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    pSLIM-N

pSLIM-N vector

cat.# FP212

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
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pSLIM-NFP21220 μg€ 400
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Vector typemammalian expression vector
ReporterSLIM
Reporter codon usagemammalian
Promoter for SLIMPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use SLIM expression in mammalian cells; generation of fusions to the SLIM N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 592-678
BrUSLEE
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1396-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1551-1556 & 1580-1585
mRNA 3' ends: 1589 & 1601
f1 single-strand DNA origin: 1648-2103
Bacterial promoter for expression of Kanr gene
-35 region: 2165-2170
-10 region: 2188-2193
Transcription start point: 2200
SV40 origin of replication: 2444-2579
SV40 early promoter
Enhancer (72-bp tandem repeats): 2277-2348 & 2349-2420
21-bp repeats: 2424-2444, 2445-2465 & 2467-2487
Early promoter element: 2500-2506
Major transcription start points: 2496, 2534, 2540 & 2545
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2628-2630
Stop codon: 3420-3422
G->A mutation to remove Pst I site: 2810
C->A (Arg to Ser) mutation to remove BssH II site: 3156
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3658-3663 & 3671-3676
pUC plasmid replication origin: 4007-4650


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SLIM-related materials (also referred to as "Products") are intended for research use only.

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