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    pPS-CFP2-N

pPS-CFP2-N vector

cat.# FP802

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pPS-CFP2-NFP80220 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReporterPS-CFP2
Reporter codon usagemammalian
Promoter for PS-CFP2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use PS-CFP2 expression in mammalian cells; generation of fusions to the PS-CFP2 N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
PS-CFP2
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1393-1395
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1549-1554 & 1578-1583
mRNA 3' ends: 1587 & 1599
f1 single-strand DNA origin: 1646-2101
Bacterial promoter for expression of Kanr gene
-35 region: 2163-2168
-10 region: 2186-2191
Transcription start point: 2198
SV40 origin of replication: 2442-2577
SV40 early promoter
Enhancer (72-bp tandem repeats): 2275-2346 & 2347-2418
21-bp repeats: 2422-2442, 2443-2463 & 2465-2485
Early promoter element: 2498-2504
Major transcription start points: 2494, 2532, 2538 & 2543
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2626-2628
Stop codon: 3418-3420
G->A mutation to remove Pst I site: 2808
C->A (Arg to Ser) mutation to remove BssH II site: 3154
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3656-3661 & 3669-3674
pUC plasmid replication origin: 4005-4648


Notice to Purchaser:

PS-CFP2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,897,726; 7,879,988; 7,667,016; European Pat. 1485481; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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