pPS-CFP2-N vector

cat.# FP802

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pPS-CFP2-N FP802 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter PS-CFP2 Reporter codon usage mammalian Promoter for PS-CFP2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use PS-CFP2 expression in mammalian cells; generation of fusions to the PS-CFP2 N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
PS-CFP2
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1393-1395
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1549-1554 & 1578-1583
mRNA 3' ends: 1587 & 1599
f1 single-strand DNA origin: 1646-2101
Bacterial promoter for expression of Kan^r gene
-35 region: 2163-2168
-10 region: 2186-2191
Transcription start point: 2198
SV40 origin of replication: 2442-2577
SV40 early promoter
Enhancer (72-bp tandem repeats): 2275-2346 & 2347-2418
21-bp repeats: 2422-2442, 2443-2463 & 2465-2485
Early promoter element: 2498-2504
Major transcription start points: 2494, 2532, 2538 & 2543
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2626-2628
Stop codon: 3418-3420
G->A mutation to remove Pst I site: 2808
C->A (Arg to Ser) mutation to remove BssH II site: 3154
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3656-3661 & 3669-3674
pUC plasmid replication origin: 4005-4648