pPS-CFP2-C vector

cat.# FP801

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pPS-CFP2-C FP801 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter PS-CFP2 Reporter codon usage mammalian Promoter for PS-CFP2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use PS-CFP2 expression in mammalian cells; generation of fusions to the PS-CFP2 C-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
PS-CFP2
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1405-1407
Last amino acid in PS-CFP2: 1324-1326
MCS: 1327-1412
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1547-1552 & 1576-1581
mRNA 3' ends: 1585 & 1597
f1 single-strand DNA origin: 1644-2099
Bacterial promoter for expression of Kan^r gene
-35 region: 2161-2166
-10 region: 2184-2189
Transcription start point: 2196
SV40 origin of replication: 2440-2575
SV40 early promoter
Enhancer (72-bp tandem repeats): 2273-2344 & 2345-2416
21-bp repeats: 2420-2440, 2441-2461 & 2463-2483
Early promoter element: 2496-2502
Major transcription start points: 2492, 2530, 2536 & 2541
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2624-2626
Stop codon: 3416-3418
G->A mutation to remove Pst I site: 2806
C->A (Arg to Ser) mutation to remove BssH II site: 3152
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3654-3659 & 3667-3672
pUC plasmid replication origin: 4003-4646