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pPA-TagRFP-H2B vector

cat.# FP815

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pPA-TagRFP-H2BFP81520 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for PA-TagRFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red fluorescent labeling of histone H2B

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
H2B-PA-TagRFP fusion: 657-1754
Histone H2B: 657-1034
PA-TagRFP: 1053-1754
Stop codon: 1752-1754
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1908-1913 & 1937-1942
mRNA 3' ends: 1946 & 1958
f1 single-strand DNA origin: 2005-2460
Bacterial promoter for expression of Kanr gene
-35 region: 2522-2527
-10 region: 2545-2550
Transcription start point: 2557
SV40 origin of replication: 2801-2936
SV40 early promoter
Enhancer (72-bp tandem repeats): 2634-2705 & 2706-2777
21-bp repeats: 2781-2801, 2802-2822 & 2824-2844
Early promoter element: 2857-2863
Major transcription start points: 2853, 2891, 2897 & 2902
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2985-2987
Stop codon: 3777-3779
G->A mutation to remove Pst I site: 3167
C->A (Arg to Ser) mutation to remove BssH II site: 3513
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4015-4020 & 4028-4033
pUC plasmid replication origin: 4364-5007

Notice to Purchaser:

PA-TagRFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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