pKillerOrange-dMito vector

cat.# FP224

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt)

Product Cat.# Size Price pKillerOrange-dMito FP224 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter KillerOrange Reporter codon usage mammalian Promoter for KillerOrange PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Expression of mitochondria-targeted KillerOrange in mammalian cells under the control of CMV promoter; source of mitochondria-targeted KillerOrange coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
KillerOrange-dMito fusion
Start codon (ATG): 597-599
Mitochondrial localization signal 1 (MLS-1): 597-689
Mitochondrial localization signal 2 (MLS-2): 690-782
Start of KillerOrange coding sequence(GAG): 801-803
Stop codon: 1509-1511
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1664-1669 & 1693-1698
mRNA 3' ends: 1702 & 1714
f1 single-strand DNA origin: 1761-2216
Bacterial promoter for expression of Kan^r gene
-35 region: 2278-2283
-10 region: 2301-2306
Transcription start point: 2313
SV40 origin of replication: 2557-2692
SV40 early promoter
Enhancer (72-bp tandem repeats): 2390-2461 & 2462-2533
21-bp repeats: 2537-2557, 2558-2578 & 2580-2600
Early promoter element: 2613-2619
Major transcription start points: 2609, 2647, 2653 & 2658
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2741-2743
Stop codon: 3533-3535
G->A mutation to remove Pst I site: 2923
C->A (Arg to Ser) mutation to remove BssH II site: 3269
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3771-3776 & 3784-3789
pUC plasmid replication origin: 4120-4763