pCasper3-GR vector

cat.# FP971

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pCasper3-GR FP971 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter Casper3-GR Reporter codon usage mammalian Promoter for Casper3-GR PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Expression of fluorescent caspase-3 apoptosis sensor Casper3-GR in mammalian cells under the control of CMV promoter; source of Casper3-GR coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Casper3-GR
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
TagRFP: 679-1374
Linker: 1375-1425
TagGFP: 1426-2130
Stop codon: 2131-2133
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2286-2291 & 2315-2320
mRNA 3' ends: 2324 & 2336
f1 single-strand DNA origin: 2383-2838
Bacterial promoter for expression of Kan^r gene
-35 region: 2900-2905
-10 region: 2923-2928
Transcription start point: 2935
SV40 origin of replication: 3179-3314
SV40 early promoter
Enhancer (72-bp tandem repeats): 3012-3083 & 3084-3155
21-bp repeats: 3159-3179, 3180-3200 & 3202-3222
Early promoter element: 3235-3241
Major transcription start points: 3231, 3269, 3275 & 3280
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3363-3365
Stop codon: 4155-4157
G->A mutation to remove Pst I site: 3545
C->A (Arg to Ser) mutation to remove BssH II site: 3891
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4393-4398 & 4406-4411
pUC plasmid replication origin: 4742-5385