TagGFP2 (scientific name mTagGFP) is the improved variant of TagGFP, the mutant of the Aequorea macrodactyla GFP-like protein [Xia et al., 2002, Subach et al., 2008]. TagGFP2 possesses bright green fluorescence with excitation/emission maxima at 483 and 506 nm, respectively.

TagGFP2 matures 1.6-fold faster than TagGFP and is characterized by the improved performance in fusions. Compared to EGFP, TagGFP2 provides about the same brightness of fluorescence but is significantly more pH stable. TagGFP2 is specially optimized for expression at 37°C.

Because of monomeric nature, TagGFP2 is mainly intended for protein localization studies and expression in long-term cell cultures. In FRET applications, TagGFP2 can be used as a donor for red fluorescent protein TagRFP or as an acceptor for blue fluorescent protein TagBFP.