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Far-red fluorescent protein TagFP635
- Bright far-red fluorescence
- Monomer with successful performance in fusions
- High photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling
Main properties
TagFP635 normalized excitation (thin line) and emission (thick line) spectra.
Download TagFP635 spectra (xls)
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
**First value – measured at pH=8.2 [Shcherbo et al., 2007], second value – measured at pH=7.5 [Shcherbo et al., 2009].
| | Molecular weight, kDa | 27 | | Polypeptide length, aa | 237 | | Fluorescence color | far-red | | Excitation maximum, nm | 588 | | Emission maximum, nm | 635 | | Quantum yield | 0.33 / 0.28 ** | | Extinction coefficient, M-1cm-1 | 45 000 / 31 500 ** | | Brightness* | 14.9 / 8.8 ** | | Brightness, % of EGFP | 45 / 26 ** | | pKa | 6.0 | | Structure | monomer | | Aggregation | no | | Maturation rate at 37°C | fast | | Photostability | high | | Cell toxicity | not observed | | Main advantages | monomeric far-red fluorescent protein, high photostability |
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 | Gel-filtration of TurboGFP (dimer, green line), EGFP (monomer, blue line), and TagFP635 (monomer, red line).
Image from Shcherbo et al., 2007.
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References:
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Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM.
Bright far-red fluorescent protein for whole-body imaging.
Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
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Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM.
Far-red fluorescent tags for protein imaging in living tissues.
Biochem J. 2009; 418 (3):567-74. doi: 10.1042/BJ20081949 / pmid: 19143658
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