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    pPS-CFP2-C

pPS-CFP2-C vector

cat.# FP801

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pPS-CFP2-CFP80120 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Vector typemammalian expression vector
ReporterPS-CFP2
Reporter codon usagemammalian
Promoter for PS-CFP2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use PS-CFP2 expression in mammalian cells; generation of fusions to the PS-CFP2 C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
PS-CFP2
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1405-1407
Last amino acid in PS-CFP2: 1324-1326
MCS: 1327-1412
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1547-1552 & 1576-1581
mRNA 3' ends: 1585 & 1597
f1 single-strand DNA origin: 1644-2099
Bacterial promoter for expression of Kanr gene
-35 region: 2161-2166
-10 region: 2184-2189
Transcription start point: 2196
SV40 origin of replication: 2440-2575
SV40 early promoter
Enhancer (72-bp tandem repeats): 2273-2344 & 2345-2416
21-bp repeats: 2420-2440, 2441-2461 & 2463-2483
Early promoter element: 2496-2502
Major transcription start points: 2492, 2530, 2536 & 2541
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2624-2626
Stop codon: 3416-3418
G->A mutation to remove Pst I site: 2806
C->A (Arg to Ser) mutation to remove BssH II site: 3152
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3654-3659 & 3667-3672
pUC plasmid replication origin: 4003-4646


Notice to Purchaser:

PS-CFP2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,897,726; 7,879,988; 7,667,016; European Pat. 1485481; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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