Tools for testing promoter activity

- Wide collection of fast-maturing fluorescent proteins
- No exogenous chemical compounds or cofactors required
- Promoterless vectors encoding destabilized fluorescent proteins available

Evrogen superbright and fast-maturing TurboFPs fluorescent proteins are specially recommended for use in applications requiring fast appearance of bright fluorescence, including in vivo tracking promoter activity and gene expression.

TurboFPs mature noticeably faster than most other fluorescent proteins and hereby allow monitoring the activity from early promoters. Ranging in color from green to far-red, TurboFPs proteins are ideal for simultaneous analysis of gene expression from different promoters in a particular cell or a cell population.

Destabilized protein variants with a rapid protein turnover allow accurate monitoring of rapid and/or transient events in gene regulation.

Please see list of promoterless vectors available

In vivo comparison of TurboGFP and EGFP maturation in developing Xenopus embryos

Vectors expressing the respective fluorescent proteins under the control of CMV promoter were microinjected into animal poles of Xenopus embryos at the stage of two blastomeres. Living embryos were then photographed from the animal pole at the middle and late gastrula stages.
Experimental data were presented by Dr. A. Zaraisky, Institute of Bioorganic Chemistry, RAS (Moscow, Russia).

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