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Cyan-to-green photoswitchable fluorescent protein PS-CFP2
- Monomer, succesful perfomance in fusions
- Irreversible photoconversion from a cyan to a green fluorescent form
- High contrast of photoconversion
- High pH stability allowing labeling of acidic organelles
- Recommended for tracking cell, organelle, and protein movement, monitoring the protein turnover and superresolution imaging
Available variants and fusions
| Variant | Description | Related vector | Cat.# |
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Humanized PS-CFP2
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PS-CFP was obtained from Aequorea coerulescens colorless GFP-like protein [Gurskaya et al., 2003]. As compared with PS-CFP, PS-CFP2 comprises S108T, M153V, and T154A substitutions, which lead to a much faster maturation and a brighter fluorescence both before and after photoswitching. PS-CFP2 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pPS-CFP2-C
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FP801
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pPS-CFP2-N
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FP802
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References:
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Gurskaya NG, Fradkov AF, Pounkova NI, Staroverov DB, Bulina ME, Yanushevich YG, Labas YA, Lukyanov S, Lukyanov KA.
A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants.
Biochem J. 2003; 373 (Pt 2):403-8. / pmid: 12693991
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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