Green fluorescent protein CopGFP
- Bright green fluorescence
- Extra fast maturation at a wide range of temperatures
CopGFP (ppluGFP2 in our scientific publications, Shagin et al., 2004) is a green fluorescent protein cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). CopGFP is characterized by superbright green fluorescence (excitation/ emission max = 482/ 502 nm) and fast maturation rate at a wide range of temperatures.
CopGFP demonstrates successful performance when expressed in cold-blooded animals, however exhibits a tendency to form aggregates in mammalian cells especially in long-term cultures.
CopGFP normalized excitation (thin line) and emission (thick line) spectra.
Download CopGFP spectra (xls)
|* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.|
|Molecular weight, kDa||26|
|Excitation maximum, nm||482|
|Emission maximum, nm||502|
|Extinction coefficient, M-1cm-1||70000|
|Brightness, % of EGFP||126|
|Maturation rate at 37°C||super fast|
|Cell toxicity||not observed|
Recommended filter sets and antibodies
CopGFP can be recognized using Anti-TurboGFP(d) (Cat.# AB513) antibodies available from Evrogen.
CopGFP can be detected using common fluorescence filter sets for EGFP, FITC, and other green dyes. Recommended Omega Optical filter sets are QMAX-Green, XF100-2, XF100-3, XF115-2, and XF116-2.
Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV.
GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity.
Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095