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    CopGFP

Green fluorescent protein CopGFP

Newer version is available, please see TurboGFP description.

 

- Bright green fluorescence
- Extra fast maturation at a wide range of temperatures

CopGFP (ppluGFP2 in our scientific publications, Shagin et al., 2004) is a green fluorescent protein cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). CopGFP is characterized by superbright green fluorescence (excitation/ emission max = 482/ 502 nm) and fast maturation rate at a wide range of temperatures.

CopGFP demonstrates successful performance when expressed in cold-blooded animals, however exhibits a tendency to form aggregates in mammalian cells especially in long-term cultures.

Main properties

CopGFP spectra

CopGFP normalized excitation (thin line) and emission (thick line) spectra.

Download CopGFP spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa26
Fluorescence colorgreen
Excitation maximum, nm482
Emission maximum, nm502
Quantum yield0.60
Extinction coefficient, M-1cm-170000
Brightness*42.0
Brightness, % of EGFP126
pKa4.3
Structuretetramer
Aggregationyes
Maturation rate at 37°Csuper fast
Photostabilityhigh
Cell toxicitynot observed

Recommended filter sets and antibodies

CopGFP can be recognized using Anti-TurboGFP(d) (Cat.# AB513) antibodies available from Evrogen.

CopGFP can be detected using common fluorescence filter sets for EGFP, FITC, and other green dyes. Recommended Omega Optical filter sets are QMAX-Green, XF100-2, XF100-3, XF115-2, and XF116-2.

References:

  • Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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