pKillerOrange-C vector

cat.# FP221

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt)

Product Cat.# Size Price pKillerOrange-C FP221 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter KillerOrange Reporter codon usage mammalian Promoter for KillerOrange PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use KillerOrange expression in mammalian cells; generation of fusions to the KillerOrange C-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
KillerOrange
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1402-1404
Last amino acid in KillerOrange: 1321-1323
MCS: 1324-1411
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1544-1549 & 1573-1578
mRNA 3' ends: 1582 & 1594
f1 single-strand DNA origin: 1641-2096
Bacterial promoter for expression of Kan^r gene
-35 region: 2158-2163
-10 region: 2181-2186
Transcription start point: 2193
SV40 origin of replication: 2437-2572
SV40 early promoter
Enhancer (72-bp tandem repeats): 2270-2341 & 2342-2413
21-bp repeats: 2417-2437, 2438-2458 & 2460-2480
Early promoter element: 2493-2499
Major transcription start points: 2489, 2527, 2533 & 2538
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2621-2623
Stop codon: 3413-3415
G->A mutation to remove Pst I site: 2803
C->A (Arg to Ser) mutation to remove BssH II site: 3149
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3651-3656 & 3664-3669
pUC plasmid replication origin: 4000-4643