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Licensing opportunities: duplex-specific nuclease

- Selectively cleaves ds DNA and DNA in DNA-RNA hybrid duplexes
- Discriminates between perfectly and nonperfectly matched short duplexes
- Inactive towards ss DNA and RNA
- Thermostable
- Inhibited by EDTA

Duplex-specific nuclease (DSN) is an enzyme purified from hepatopancreas of Red King (Kamchatka) crab (Shagin et al., 2002). DSN shows a strong preference for cleaving double-stranded (ds) DNA and DNA in DNA-RNA hybrid duplexes, and is practically inactive towards single-stranded (ss) DNA or single or double-stranded RNA. Moreover, the enzyme is capable to discriminate between perfectly and nonperfectly matched short DNA duplexes. DSN is widely used for full-length enriched cDNA normalization (Zhulidov et al., 2004; Bogdanova et al., 2011a) and for construction of normalized RNA-seq libraries for next generation sequencing ((Christodoulou et al., 2011), Illumina DSN normalization protocol). The list of approved DSN applications also includes normalization of genomic DNA (Shagina et al., 2010), cDNA depletion and ribosomal cDNA depletion (Bogdanova et al., 2009; Bogdanova et al., 2011b; Yi et al., 2011), cDNA subtraction (Peng et al., 2008), SNP detection (Shagin et al., 2002; Liu et al., 2011), construction of repeat-free FISH probes (Swennenhuis et al., 2012), multiplexed fluorescence detection of miRNAs (Yin et al., 2012), quantitative telomeric overhang determination (Zhao et al., 2008; Zhao et al., 2011), etc.

Evrogen technology embodied in DSN is available for commercial use through an adaptable licensing program. The licensing options may include development of novel products and applications.

Evaluation of the technologies is easily available by purchase of the related research use product. Please see "Notice to Purchaser" section for the product of your interest for details.

For license information please contact Evrogen by e-mail at license@evrogen.com.

Related publications:

  • Bogdanova EA, Shagin DA, Lukyanov SA. Normalization of full-length enriched cDNA. Mol Biosyst. 2008; 4 (3):205-12. / pmid: 18437263
  • Cheung F, Haas BJ, Goldberg SM, May GD, Xiao Y, Town CD. Sequencing Medicago truncatula expressed sequenced tags using 454 Life Sciences technology. BMC Genomics. 2006 Oct 24;7:272. / pmid: 17062153
  • Deng W, Zhu X, Skogerbo G, Zhao Y, Fu Z, Wang Y, He H, Cai L, Sun H, Liu C, Li B, Bai B, Wang J, Jia D, Sun S, He H, Cui Y, Wang Y, Bu D, Chen R. Organization of the Caenorhabditis elegans small non-coding transcriptome: genomic features, biogenesis, and expression. Genome Res. 2006 Jan;16(1):20-9. / pmid: 16344563
  • Du C, Ge B, Liu Z, Fu K, Chan WC, McKeithan TW. PCR-based generation of shRNA libraries from cDNAs. BMC Biotechnol. 2006 Jun 21;6:28. / pmid: 16790063
  • Shagin DA, Rebrikov DV, Kozhemyako VB, Altshuler IM, Shcheglov AS, Zhulidov PA, Bogdanova EA, Staroverov DB, Rasskazov VA, Lukyanov S. A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas. Genome Res. 2002; 12 (12):1935-42. / pmid: 12466298
  • Shcheglov A, Zhulidov P, Bogdanova E, Shagin D. Normalization of cDNA Libraries. In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) ISBN: 978-1-4020-6039-7. 2007; 97-124. http://www.springerlink.com
  • Zhao Y, Hoshiyama H, Shay JW, Wright WE. Quantitative telomeric overhang determination using a double-strand specific nuclease. Nucleic Acids Res. 2008; 36 (3):e14. / pmid: 18073199
  • Zhulidov PA, Bogdanova EA, Shcheglov AS, Vagner LL, Khaspekov GL, Kozhemyako VB, Matz MV, Meleshkevitch E, Moroz LL, Lukyanov SA, Shagin DA. Simple cDNA normalization using kamchatka crab duplex-specific nuclease. Nucleic Acids Res. 2004; 32 (3):e37. / pmid: 14973331
  • Zhulidov PA, Bogdanova EA, Shcheglov AS, Shagina IA, Wagner LL, Khazpekov GL, Kozhemyako VV, Lukyanov SA, Shagin DA. A method for the preparation of normalized cDNA libraries enriched with full-length sequences. Bioorg Khim. 2005; 31 (2):186-94. http://www.springerlink.com / pmid: 15889793
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