pKillerOrange-N vector

cat.# FP222

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt)

Product Cat.# Size Price pKillerOrange-N FP222 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter KillerOrange Reporter codon usage mammalian Promoter for KillerOrange PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use KillerOrange expression in mammalian cells; generation of fusions to the KillerOrange N-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-678
KillerOrange
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1390-1392
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1545-1550 & 1574-1579
mRNA 3' ends: 1583 & 1595
f1 single-strand DNA origin: 1642-2097
Bacterial promoter for expression of Kan^r gene
-35 region: 2159-2164
-10 region: 2182-2187
Transcription start point: 2194
SV40 origin of replication: 2438-2573
SV40 early promoter
Enhancer (72-bp tandem repeats): 2271-2342 & 2343-2414
21-bp repeats: 2418-2438, 2439-2459 & 2461-2481
Early promoter element: 2494-2500
Major transcription start points: 2490, 2528, 2534 & 2539
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2622-2624
Stop codon: 3414-3416
G->A mutation to remove Pst I site: 2804
C->A (Arg to Ser) mutation to remove BssH II site: 3150
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3652-3657 & 3665-3670
pUC plasmid replication origin: 4001-4644