	Tersus polymerase provides highly specific amplification of difficult templates. A 230 bp fragment of L1Hs repeat was amplified from 5 ng of human genomic DNA using different polymerases. M - 1 kb DNA ladder; 1 – Taq polymerase; 2 – Encyclo polymerase; 3 – Tersus polymerase, 28 cycles.

Tersus polymerase provides highly specific amplification of difficult templates.

A 230 bp fragment of L1Hs repeat was amplified from 5 ng of human genomic DNA using different polymerases.
M - 1 kb DNA ladder; 1 – Taq polymerase; 2 – Encyclo polymerase; 3 – Tersus polymerase, 28 cycles.

	PCR amplification of phage λ DNA fragments using Tersus polymerase. DNA fragments were amplified from 25 ng of template in 15 cycles (wells 1 and 2) and 19 cycles (wells 3-5). 1 – 3 kb fragment; 2 – 5 kb fragment; 3 – 8 kb fragment; 4 – 10 kb fragment; 5 – 15 kb fragment; M – 1 kb DNA ladder.

PCR amplification of phage λ DNA fragments using Tersus polymerase.

DNA fragments were amplified from 25 ng of template in 15 cycles (wells 1 and 2) and 19 cycles (wells 3-5).
1 – 3 kb fragment; 2 – 5 kb fragment; 3 – 8 kb fragment; 4 – 10 kb fragment; 5 – 15 kb fragment; M – 1 kb DNA ladder.

Possible limitations:

- Not recommended for amplification of long (>3 kb) templates (use Encyclo polymerase instead) - Not suitable for real-time PCR methods based on DNA probe breakdown (such as TaqMan) due to the lack of 5’>3’ exonuclease activity - Not recommended for allele-specific SNP detection due to the presence of proofreading 3’>5’ exonuclease activity