Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
mKate2
Start codon (ATG): 613-615
Last amino acid in mKate2: 1312-1314
Farnesylation signal: 1330-1389
Stop codon: 1390-1392
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1584-1589 & 1613-1618
mRNA 3' ends: 1622 & 1634
f1 single-strand DNA origin: 1681-2136
Bacterial promoter for expression of Kan^r gene
-35 region: 2198-2203
-10 region: 2221-2226
Transcription start point: 2233
SV40 origin of replication: 2477-2612
SV40 early promoter
Enhancer (72-bp tandem repeats): 2310-2381 & 2382-2453
21-bp repeats: 2457-2477, 2478-2498 & 2500-2520
Early promoter element: 2533-2539
Major transcription start points: 2529, 2567, 2573 & 2578
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2661-2663
Stop codon: 3453-3455
G->A mutation to remove Pst I site: 2843
C->A (Arg to Ser) mutation to remove BssH II site: 3189
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3691-3696 & 3704-3709
pUC plasmid replication origin: 4040-4683