Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
mKate2-clathrin fusion: 613-1992
mKate2: 613-1314
Start codon (ATG): 613-615
Last amino acid in mKate2: 1312-1314
Clathrin LCB: 1360-1992
Stop codon: 1993-1995
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2156-2161 & 2185-2190
mRNA 3' ends: 2194 & 2206
f1 single-strand DNA origin: 2253-2708
Bacterial promoter for expression of Kan^r gene
-35 region: 2770-2775
-10 region: 2793-2798
Transcription start point: 2805
SV40 origin of replication: 3049-3184
SV40 early promoter
Enhancer (72-bp tandem repeats): 2882-2953 & 2954-3025
21-bp repeats: 3029-3049, 3050-3070 & 3072-3092
Early promoter element: 3105-3111
Major transcription start points: 3101, 3139, 3145 & 3150
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3233-3235
Stop codon: 4025-4027
G->A mutation to remove Pst I site: 3415
C->A (Arg to Ser) mutation to remove BssH II site: 3761
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4263-4268 & 4276-4281
pUC plasmid replication origin: 4612-5255