| mKate2 | Bgl II | Sac I | EcoR I | Sal I | Kpn I | Apa I | BamH I | STOPs | |||||||||||||||||||||||||
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| BspE I | Xho I | Hind III | Pst I | Sac II | Xba I# | Bcl I# | |||||||||||||||||||||||||||
| ... | GGT.GGA.GGA.GGT. | TCC.GGA | .CTC. | AGA. | T | CT. | C | GA.G | CT.C | AA.GCT.T | C | G.AAT.T | C | T.GCA. | G | TC.GAC. | GGT.A | CC | .GC | G.G | G | C.CC | G. | GG | A.TCC | .ACC.GGA. | TC | T.AG | A. | TAA | .C | TG.A | TC.A |
# – sites are blocked by dam methylation. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam- host and make fresh DNA.