Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboYFP-dest1
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Last amino acid in TurboYFP: 1405-1407
Amino acid residues of mouse ornithine decarboxylase (MODC) PEST sequence: 1429-1548
Stop codon: 1549-1551
Fragment of human beta globin (HBB) gene
Last 35 bp of HBB exon 2: 1560-1594
HBB intron 2: 1595-2445
First 233 bp of HBB exon 3: 2446-2678
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2820-2825 & 2849-2854
mRNA 3' ends: 2858 & 2870
f1 single-strand DNA origin: 2917-3372
Bacterial promoter for expression of Kan^r gene
-35 region: 3434-3439
-10 region: 3457-3462
Transcription start point: 3469
SV40 origin of replication: 3713-3848
SV40 early promoter
Enhancer (72-bp tandem repeats): 3546-3617 & 3618-3689
21-bp repeats: 3693-3713, 3714-3734 & 3736-3756
Early promoter element: 3769-3775
Major transcription start points: 3765, 3803, 3809 & 3814
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3897-3899
Stop codon: 4689-4691
G->A mutation to remove Pst I site: 4079
C->A (Arg to Ser) mutation to remove BssH II site: 4425
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4927-4932 & 4940-4945
pUC plasmid replication origin: 5276-5919